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Format:Sterilefiltered,lyophilized.

Purification:Chromatographic

RecombinantHumanLeptinproducedin

E.coliisasingle,non-glycosylated,polypeptidechaincontaining147aminoacidswithamolecularmassof16,240daltons.ThisproducthasfullBIOLOGicalactivity.

Source:EscherichiacoliStABIlizers:NonePreservatives:None

Purity:Greaterthan95%byRP-HPLC,anion-exchangechromatography;reducingandnon-reducingSDS-PAGE,silverstain.

Dimers/Aggregates:<1%bySDS-PAGE

BiologicalActivity:Fullybiologicallyactivecomparedtoreferencestandards.ED50determinedbytheleptin-

dependentstimulationofhumanOB-RtransfectedmurineBaF3cellsis0.5-1.6ng/ml.

Endotoxin:<0.1ng/ug

ProteinContent:Determinedspectrophotometricallyat280nm(extinctioncoefficient0.878)andbyRP-HPLCanalysisagainstacalibratedstandard.

Solubleinwaterandmostaqueousbuffersaboveandbelowtheisoelectricpoint.Dialyzedagainst45mMsodiumbicarbonatepriortolyophilization.

Lyophilizedproductisstableatroomtemperature,butshouldbestoreddesiccatedat-20°C.Uponreconstitution,storeat4°C.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures

QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

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qedbio.com

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。