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CaMKII,amemberofthecalcium/calmodulin-activatedproteinkinasfamily,functionsinneuralsynapaticstimulationandT-cellreceptorsignaling.CaMKIIisexpressedinmanytissues,butitisspecificallyfoundintheneuronsoftheforebrain,anditsmRNAisfoundwithinthedendritesandthesomaoftheneuron.

CaMKIIinneuronsconsistsoftwosubunitsof52(alphagenes)and60kDa(betagenes).CaMKIIhascatalyticandregulatorydomainsaswellasanATP-bindingdomainandaconsensusphosphorylationsite.

Bindingofcalcium/calmodulintoitsregulatorydomainreleasesitsauto-inhibitoryeffectandactivatesthekinase.Thiskinaseactivationresultsinautophosphorylationatthreonine286.

AutophosphorylationconfersenhancedaffinityofCaMKIIforNMDAreceptorsinpostsynapticdensities.

Thisantibodyrecognizesthephosphorylatedandnon-phosphorylatedformsofmouse,rat,andbovineCaMKII.

Immunoblotting:useat0.1-1ug/ml.Abandof~50-60kDaisdetected.

Immunohistochemistry:useat10ug/ml.

RIA:useat3-5ug/ml.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Ratbraintissueextract.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。