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CatalogNo.:12530(cloneS117-9)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

MethylationofDNAatcytosineresiduesisimportantinregulationofgeneexpression.HypermethylationofCpGislandsintumorsuppressorgenesorhypomethylationofbulkgenomicDNAmaybelinkedtodevelopmentofcancer.ThreefamiliesofmammalianDNAmethyltransferasegeneshavebeenidentified:DNMT1,DNMT2,andDNMT3.TheDNMT3familymembers,DNMT3aandDNMT3b,arestronglyexpressedinembryonicstem(ES)cells,buttheirexpressionisdownregulatedindifferentiatingEScellsandislowinadultsomatictissue.StudiesshowthatDNMT3LregulatestheactivityofDNMT3aandDNMT3bandstimulatestheircatalyticactivities.DNMT3Lhasbeenlinkedspecificallytotheprocessofcarcino-genesisthroughitsroleinnuclearprogramming.

BAA95556).

ThisantibodyrecognizeshumanDNMT3L.Itdoesnotcross-reactwithDNMT3aorDNMT3b.

Immunoblotting:useat1-10ug/ml.Abandof~45kDaisdetected.

ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:LysateofCOScellstransientlytransfectedwithFlag-taggedDNMT3L.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。