CatalogNo.:12595(clone4G11-C7)Size:100uginPBS(2mg/ml),pH7.4,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

MethylationofDNAatcytosineresiduesisimportantinregulationofgeneexpressionandgenomicimprinting.Hyper-methylationofCpGislandsintumorsuppressorgenesorhypomethylationofbulkgenomicDNAmaybelinkedtodevelopmentofcancer.ThusfarthreefamiliesofmammalianDNAmethyl-transferasegeneshavebeenidentified:DNMT1,DNMT2,andDNMT3.DNMT1is

constitutivelyexpressedinproliferatingcells,andinactivationofthisgenecausesglobaldemethylationofgenomicDNAandembryoniclethality.DNMT1co-purifieswiththeretinoblastoma(Rb)tumorsuppressorgeneproductE2F1andwithHDAC1.DNMT1cooperateswithRbtorepresstranscriptionfrompromoterscontainingE2F-bindingsiteswhichsuggestsalinkbetweenDNAmethylation,histonedeacetylaseandsequence-specificDNAbindingactivityaswellasagrowth-regulatorypathwaythatisdisruptedincancercells.

Antigen:Syntheticpeptidecorrespondingtoaa637-650ofhumanDNMT1(accessionno.NP_001370).HostSpecies:Mouse

AntibodyClass:IgG2b

Thisantibodyrecognizeshuman,mouse,andZebrafishDNMT1.

ELISA

Immunoblotting:useat2ug/ml.Abandof

~190kDaisdetected.

Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrols:ESandH1299celllysates.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。