ORDERINGINFORMATION

Format:ProteinG-purifiedantibodyinPBS,pH7.4.

BACKGROUND

Ethidiumbromide(EB)foundfameinthelate1940sasanantitrypanosomal,antimicrobial,antibacterial,andantiviralagent.ItsBIOLOGicaleffectsareadirectconsequenceoftheinhibitionofnucleicacidsynthesis,whichinturnisrelatedtothespecificbindingofthedrugtoDNA.EBinhibitsDNApolymeraseandbindsinvitrotobothRNAandDNA.InvestigationintotheprecisenatureoftheDNA-EBbindingmechanismledtothediscoverythatEBbindsbyamechanismtermedintercalation.Thisprocesshasbeenstudiedextensivelyduringthepastthreedecades,andthephotophysicalchangesthataccompanyintercalationhavebeensuccessfullyappliedtoquantitateandstructurallyelucidateDNA.MorerelevanttotheuseofEBinmolecularbiologyaretheobservedvariationsinitsfluorescentcharacteristicsonbindingtopolynucleotides.

SPECIFICATIONSUMMARY

Antigen:EthidiumbromideintercalatedcalfthymusDNA.

HostSpecies:Mouse

Specificity:TheseantibodiesspecificallyrecognizeintercalatedeukaryoticDNAanddonotcross-reactwithsingleordouble-strandednon-intercalatedDNAorwithethidiumbromide.

APPLICATIONS

TheseantibodiesmaybeusedinELISAtodetectandquantitateintercalatedDNA.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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