ORDERINGINFORMATION
CatalogNo.:43034
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Myc(c-Myc)isaregulatorgenethatcodesfortranscriptionfactorMyc.Thisproteinisamultifunctional,nuclearphosphoproteinthatplaysaroleincellcycleprogression,apoptosisandcellulartransformation.AmutatedversionofMycisfoundinmanycancerswhichcausesMyctobeconstitutivelyexpressed.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine58(L-P-T(p)-P-P)derivedfromhumanMycandconjugatedtoKLH.
Accessionno.:P01106,NP_002458.2
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratMyconlywhenphosphorylatedatthreonine58.
APPLICATION
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~60kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofMyc(phospho-Thr58)inextractsofHeLacells(1)afterpre-incubationof#43034withblockingpeptideor(2)withnopretreatmentofantibody.
DetectionofMyc(phospho-Thr58)inparaffin-embeddedhumanbreastcarcinomatissue.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
美国QED Bioscience 公司(www.qedbio.com)是专业的抗体公司,有20年的历史,是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司,能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。