ORDERINGINFORMATION
CatalogNo.:43040
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
Theprocessofdifferentiationfrommesodermalprecursorcellstomyoblastshasledtothediscoveryofavarietyoftissue-specificfactorsthatregulatemusclegeneexpressionincludingtheDNAbindingregulatoryproteinmyocyte-specificenhancerfactor-2(MEF2)family.EachoftheseproteinsbindstotheMEF2targetDNAsequencepresentintheregulatoryregionsofmanymuscle-specificgenes.TheMEF2genesaremembersoftheMADSgenefamilythatalsoincludesseveralhomeoticgenesandothertranscriptionfactorsallofwhichshareaconservedDNA-bindingdomain.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofthreonine319(V-T-T(p)-P-S)derivedfromhumanMEF2aandconjugatedtoKLH.
Accessionno.:Q02078,NP_001124398.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratMEF2aonlywhenphosphorylatedatthreonine319.
APPLICATION
Immunofluorescence:useatdilutionof1:100-1:200.
DetectionofMEF2a(phospho-Thr319)inmethanol-fixedHeLacells.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
美国QED Bioscience 公司(www.qedbio.com)是专业的抗体公司,有20年的历史,是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司,能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。