ORDERINGINFORMATION
CatalogNo.:43028
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
JunDproteinencodedbythisintronlessgeneisamemberoftheJUNfamilyandafunctionalcomponentoftheAP1transcriptionfactorcomplex.Thisproteinhasbeenproposedtoprotectcellsfromp53-dependentsenescenceandapoptosis.Alternativetranslationinitiationsiteusageresultsintheproductionofdifferentisoforms.
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine255(G-E-S(p)-P-P)derivedfromhumanJunDandconjugatedtoKLH.
Accessionno.:P17535,NP_005345.3
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratJunDonlywhenphosphorylatedatserine255.
APPLICATION
Immunoblotting:useatdilutionof1:500-1:1,000.Abandof~38kDaisdetected.
Immunohistochemistry:useatdilutionof1:50-1:100.
DetectionofJunD(phospho-Ser255)inextractsof293cellswithantibodypre-incubatedwithblockingpeptide(+)orwithoutblockingpeptide(-).
DetectionofJunD(phospho-Ser255)inparaffin-embeddedhumanbreastcarcinomatissue.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
美国QED Bioscience 公司(www.qedbio.com)是专业的抗体公司,有20年的历史,是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司,能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。