ORDERINGINFORMATION

CatalogNo.:43043

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Hepatocytenuclearfactor4alpha(HNF4α)isanucleartranscriptionfactorthatbindsDNAasahomodimerandcontrolstheexpressionofseveralgenesincludinghepatocytenuclearfactor1,atranscriptonfactorthatregulatesexpressionofseveralhepaticgenes.HNF4αhasbeenimplicatedindevelopmentofliver,kidney,andintestines.MutationsintheHNF4αgenehavebeenassociatedwithmonogenicautosomal-dominantnon-insulin-dependentdiabetesmellitustype1.Alternativesplicingofthisgeneresultsinmultipletranscriptvariants.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine304(L-R-S(p)-Q-V)derivedfromhumanHNF4αandconjugatedtoKLH.

Accessionno.:P41235,NP_000448.3

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratHNF4αonlywhenphosphorylatedatserine304.

APPLICATION

Immunoblotting:useatdilutionof1:1,000.Abandof~52kDaisdetected.

DetectionofHNF4α(phospho-Ser304)inextractsofHeLacellstreatedoruntreatedwithAnisomycin.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。