ORDERINGINFORMATION

CatalogNo.:43004

Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.

BACKGROUND

Elk-1isamemberoftheEtsfamilyoftranscriptionfactorsandtheternarycomplexfactor(TCF)subfamily.Proteinsofthatsubfamilyformaternarycomplexbybindingtotheserumresponsefactorandtheserumresponseelementinthepromoterofthec-fosprotooncogene.Elk-1proteinisanucleartargetfortheras-raf-MAPKsignalingcascade.

SPECIFICATIONSUMMARY

Antigen:Peptidesequencethatincludesphosphorylationsiteofserine383(T-L-S(p)-P-I)derivedfromhumanElk-1andconjugatedtoKLH.

Accessionno.:P19419,NP_001107595.1

HostSpecies:Rabbit

Specificity:Thisantibodydetectsendogenoushuman,mouse,andratElk-1onlywhenphosphorylatedatserine383.

APPLICATIONS

Westernblotting:useatdilutionof1:500-1:1,000.Abandof~47kDaisdetected.

Immunohistochemistry:useatdilutionof1:50-1:100.

DetectionofElk-1(Phospho-Ser383)inHT29cellextractsuntreatedortreatedwithUV.

Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.

DetectionofElk-1(phospho-Ser383)inparaffin-embeddedhumanbreastcarcinomatissue.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-term.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。