VAMP1,2/SynaptobrevinMonoclonalAntibody
VAMP-1(Vesicle-AssociatedMembraneProtein)isan18kDamemberofthesynaptobrevinfamily.Itisexpressedinneurons,neutrophils,andskeletalmusclecellsandparticipatesinvesiclefusionwiththeplasmamembrane.Synaptobrevins,syntaxins,SNAP25arethemaincomponentsofaproteincomplexinvolvedinthedockingand/orfusionofsynapticvesicleswiththepresynapticmembrane.
VAMP-1isalsooneofthekeyproteinsintheSNAREcomplexinvolvedinregulatedexocytosis.
ThisantibodyrecognizeshumanVAMP1andVAMP2.
MarshakSetal.2012.PLoSOne7:e33153.Used#56473inimmuno-fluorescenceat1ug/ml.
APPLICATIONSImmunoblotting:useat0.5-1.0ug/ml.Abandof~18kDaisdetected.
WesternblotanalysisofVAMP1/2inratbrainwith
#56473at1ug/ml
Immunohistochemistry:useat1-5ug/ml.
Stainingofhumanbraincerebellumneuronsandfibreswith#56473
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Humanbrainlysate
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseindiagnosticortherapeuticprocedures.
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