Measlesvirus(MeV)isanenvelopedRNAvirusintheMorbillivirusgenusoftheParamyxoviridaefamily.Itsnon-segmented,negative-sense,single-strandedRNAgenomeisencapsidatedbytheviralnucleoprotein(N)withinahelicalnucleocapsid.TranscriptionandreplicationarecarriedoutonthisN:RNAcomplexbytheviralpolymerasecomplexwhichconsistsoftwocomponents,thelargeprotein(L)andthephosphoprotein(P).MeVNconsistsoftworegions:astructuredN-terminalmoiety,NCORE(aa1–400),whichcontainsalltheregionsnecessaryforself-assemblyandRNA-binding,andaC-terminaldomain,NTAIL(aa401–525)thatisintrinsicallyunstructuredandisexposedatthesurfaceoftheviralnucleocapsid.

ThisantibodyreactswithMeaslesvirusnucleoprotein.

ELISA:useat0.1-1.0ug/ml.Immunoblotting:useat1-5ug/ml.Abandof~60kDaisdetected.

Immunocytochemistry:useat2-10ug/mlonvirus-infectedcells.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Reconstituteindistilledwater.

Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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