ORDERINGINFORMATION
CatalogNo.:50114CloneNo.:OC4-30Size:100ug
Format:Antibodywaspurifiedbycolumnchromatography,dissolvedin10mMPBS,pH7.4,containing1.0%bovineserumalbumin,andthenlyophilized.Thelyophilizedantibodydoesnotcontainpreservative.
BACKGROUND
Osteocalcinisanoncollagenous,49aminoacidglutamate-richpolypeptidebonematrixproteinwithamolecularweightofabout5800Da.Osteoblastsproduceosteocalcinandincorporateitintothebonematrix.Osteocalcinisreleasedintothecirculationfromthematrixduringboneresorptionand,therefore,isconsideredaMarkerofboneturnoverratherthanaspecificmarkerofboneformation.
SPECIFICATIONSUMMARY
Antigen:BovineosteocalcinHostSpecies:MouseAntibodySubtype:IgG2a
Specificity:Theγ-carboxylatedresidueatposition17;human,bovine,rat,rabbit,pig,dog,goat,sheep,andchicken.
APPLICATIONS
SandwichELISA:1-10ug/mlwithimmobilizedantigen,pairwithcatalogno.50120.
Immunoblotting:1-10ug/ml,reducingornon-reducingconditions.
Immunohistochemistry:1-10ug/ml,frozenorparaffin-embeddedtissueafterproteinaseK(0.4m/ml)antigenretrieval.
ChondromaSynovialSarcomaSynovialSarcoma
Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
Dissolvelyophilizedantibodyin50ulofdistilledwater(2.0mg/mlfinalconcentration)thendilutefurtherforapplicationsinPBSormediathatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Storestocksolution(2.0mg/ml)inaliquotsat-20℃for1year,orat4℃for6monthsafteradding0.1%sodiumazide.Avoidrepeatedfreeze-thawcycles.Dilutedantibodyshouldnotbestored.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
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