ORDERINGINFORMATION

CatalogNo.:26108

Format:100ugpeptideaffinity-purifiedrabbitIgG(0.2mg/ml)

BACKGROUND

ChromograninB(CgB,secretograninI)isasecretorygranulematrixproteinexpressedinawidevarietyofendocrinecellsandneuronswhereitmayhavebothextracellularandintracellularfunctions.ExtracellularrolesforCgBaredependentonitsproteolyticprocessingwithinsecretorygranulestoformsmallerpeptidesthatmayhavearoleintheneuroendocrine/sympathoadrenalstressresponsetosystemicinfection,andCgBfragmentsmayalsohaveanautocrineinhibitoryroleinthereleaseofco-storedhormones,suchasinsulin.

SPECIFICATIONSUMMARY

Antigen:SyntheticpeptidederivedfromtheC-terminus(aa641-655)ofCgB(accessionno.NP_001810.Thissequenceisidenticalinhuman,mouse,andratCgB.

HostSpecies:Rabbit

Specificity:Thisantibodyrecognizeshuman,mouse,andratchromograninB.

APPLICATIONS

Immunoblotting:useat0.1-1.0ug/ml

PC12cellextract(ug)2.51.0

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

AlthoughthepredictedmwofCgBis~76kDa,thehighlyacidicnatureofCgBcausesittorunatahighermwinSDS-PAGE.

Immunohistochemistry:useat1-5ug/ml.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oCto-70oC.Avoidmultiplefreeze-thawcycles.

PRODUCTREFERENCE

ThompsonMEetal.1992.ProlactingranulogenesisisassociatedwithincreasedsecretograninexpressionandaggregationintheGolgiapparatusofGh4C1cells.Endocrinology131:318-326.Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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