Hsp90andthe94kDaglucose-regulatedprotein,Grp94,aremajormolecularchaperonesofthecytosolandendoplasmicreticulum.Inmammaliancells,thereareatleasttwoHsp90isoforms,Hsp90αandHsp90β,whichareencodedbyseparategenes.AllknownmembersoftheHsp90familyarehighlyconserved,especiallyintheN-terminalandC-terminalregions.Intheabsenceofstress,Hsp90isanessentialcomponentofcellularprocessessuchashormonesignalingandcellcyclecontrol.

Severalregulatoryproteinssuchassteroidreceptors,cellcyclekinasesandp53havebeenidentifiedassubstratesofHsp90.

Thisantibodyrecognizeshuman,mouse,andrabbitHsp90αandHsp90β(90kDa).

BarentRLetal.1998MolEndocrinol12:342;FeltsSJetal.2000JBiolChem275:3305;ArlanderSJetal.2003JBiolChem278:52572.

Immunoblotting:useat1-5ug/ml.Abandof

~90kDaisdetected.

DetectionofHsp90intumorcelllinesandratbrainwith

#11098at1ug/ml.

DetectionofHsp90inHeLacelllysatewith#11098at1ug/ml.

Immunohistochemistry:useat1-5ug/ml.Immunoprecipitation:10ugon10ulProteinA-Sepharose+100ulsample.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Heat-shockedHeLacelllysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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