Hsp70/Hsc70(Plant)MonoclonalAntibody
PurifiedbyProteinGaffinitychromatography.
BACKGROUNDHsp70genesencodeheat-inducIBLe70kDaheatshockproteins.Hsp70bindsATPwithhighaffinityandpossessesweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides.ThestructureoftheATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins.ThebindingofATPtriggersacriticalconformationalchangewhichleadstothereleaseoftheboundsubstrateprotein.TheuniversalABIlityofHsp70stoundergocyclesofbindingtoandreleasefrompartiallyunfoldedproteinsisthebasisoftheirroleinproteinsynthesis,proteinfolding,oligomerization,andproteintransport.
Antigen:PurifiedHsp70fromPhaseolusaureus(mungbean).HostSpecies:MouseAntibodyClass:IgG1
SPECIFICITYThisantibodyrecognizesconstitutiveandinducibleplantHsp70.Itdoesnotcross-reactwithhuman,rat,bacteria(DNAKK)orhumanBIP.
APPLICATIONSImmunoblotting:useat1ug/ml.Abandof
~70kDaisdetected.
WesternblotdetectionofHsp70inDuckweed(L.minor)cellswith#11138.
ELISA:useat1-10ug/ml.
Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
美国QED Bioscience 公司(www.qedbio.com)是专业的抗体公司,有20年的历史,是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司,能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。