CatalogNo.:11139(clone1.86)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

Hsp70genesencodeheat-inducIBLe70kDaheatshockproteins.Hsp70bindsATPwithhighaffinityandpossessesweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides.ThestructureoftheATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins.ThebindingofATPtriggersacriticalconformationalchangewhichleadstothereleaseoftheboundsubstrateprotein.TheuniversalABIlityofHsp70stoundergocyclesofbindingtoandreleasefrompartiallyunfoldedproteinsisthebasisoftheirroleinproteinsynthesis,proteinfolding,oligomerization,andproteintransport.

Antigen:PurifiedbovineHsp70(accessionno.NP_776975.1).

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizesinduciblebovine,human,mouseandratHsp70.Itdoesnotcross-reactwithHsc70.

Immunoblotting:useat1ug/ml.Abandof

~70kDaisdetected.

ELISA:useat1-10ug/mlwithHsp70sampleonthesolidphase.

Positivecontrol:HeLacelllysate.

Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.

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