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Hsp70bindsATPwithhighaffinityandpossessesaweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides.Hsp70recognizesandbindstonascentpolypeptidechainsaswellaspartiallyfoldedintermediatesofproteinspreventingtheiraggregationandmisfolding.ThebindingofATPtriggersacriticalconformationalchangeleADIngtothereleaseoftheboundprotein.TheABIlityofHsp70toundergocyclesofbindingandreleasefromhydrophobicstretchesofpartiallyunfoldedproteinsisthebasisfortheirroleinavarietyofintracellularfunctionssuchasproteinsynthesis,proteinfoldingandoligomerizationandproteintransport.

Antigen:RecombinanthumanHsp70expressedinE.coli.

Thisantibodyrecognizeshuman,mouse,rat,fish,avian,rabbit,amphibian,Drosophila,andyeastHsp70,Hsc70,Grp78,and,followingheatshock,Hsp72.

Immunoblotting:useat1ug/ml(ECL).Abandof~70kDaisdetected.

Immunoprecipitation:useat1ug/mlImmunohistochemistry/Immuno-fluorescence:useat2ug/ml

Usershoulddetermineoptimalconcentrationsfortheirapplications.Positivecontrol:Heat-shockedHeLacelllysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone

(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

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qedbio.com

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。