Hsp70genes,membersofamultigenefamily,encodeheat-inducIBLe70kDaheat-shockproteinsthatfunctionasmolecularchaperones.Theyhavebeenidentifiedinmostorganellesofeukaryoticcellsaswellasinbacteria.TheN-terminusofHsp70bindsATPwithhighaffinityandtheC-terminusbindsproteinsandpolypeptides.Hsp70sbindnascentpolypeptidesaswellaspartiallyfoldedintermediatesofproteins,thuspreventingtheiraggregationand/ormisfolding.BindingofATPtriggersaconformationalchangethatresultsinthereleaseofboundprotein.Hsp70sfunctioninproteinsynthesis,proteinfoldingandoligomerization,andproteintransport.

ThisantibodyrecognizesHsp70ofhuman,mouse,rat,rabbit,monkey,guineapig,hamster,chicken,bovine,canine,ovine,porcine,fish,Drosophila,andC.elegansanddoesnotcross-reactwithHsc70.Theepitopeisintheregionofaminoacids436-503ofhumanHsp70.

Immunoblotting:1ug/ml.Abandof70kDaisdetected.

ImmunoblottingofHsp70incelllysatesfromhumancancercelllineswith#11096.

Immunohistochemistry:1-10ug/ml

Immunohistochemicalstainingofinflammatorycellsinhumancolontissuewith#11096.

Immunoprecipitation:1-10ug/ml

ELISA:1-10ug/ml

Flowcytometry:10ug/ml

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:HeLacelllysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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