ORDERINGINFORMATION

CatalogNo.Size

188491mg

18849-01100ug

Format:1mg/mlinPhosphateBufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

TheHAepitopetagisderivedfromtheHA-moleculecorrespondingtoaminoacidsYPYDVPDYAofhumaninfluenzahemagglutinin.Itisfrequentlyusedasanepitopetaginexpressionvectors.ManyrecombinantproteinshavebeenengineeredtoexpresstheHAtag,whichdoesnotappeartointerferewiththebioactivityorthebiodistributionoftherecombinantprotein.

SPECIFICATIONSUMMARY

Antigen:HA(YPYDVPDYA)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:10,000-1:25,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunoprecipitation:use2-10ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofHA-taggedfusionproteinin200,67,22,7,and2ngofE.colicelllysatewith18849diluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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