ORDERINGINFORMATION

CatalogNo.Size

188321mg

18832-01100ug

Format:1mg/mlinPhosphateBufferedSaline(PBS),0.09%sodiumazide.

BACKGROUND

Enterokinase,alsoknownasenteropeptidase,isaserineproteasethatcatalyzesthehydrolysisofpeptidebondsinproteinsbut,unlikeotherkinases,doesnotcatalyzetransferofphosphategroups.Enterokinasecleavesproteinsfollowingalysineatanenterokinasecleavagesite,orECS(Asp-Asp-Asp-Asp-Lys).Invivo,thiscleavageresultsintrypsin-dependentactivationofotherpancreaticzymogens,suchaschymotrypsinogen,proelastase,procarboxypeptidaseandprolipaseinthelumenofthegut.

SPECIFICATIONSUMMARY

Antigen:DDDDKconjugatedtoKLH.

HostSpecies:Rabbit

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

Immunoprecipitation:use1-4ug/mglysate.ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DetectionofECS-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18832diluted1:25,000.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。