ORDERINGINFORMATION
CatalogNo.Size
188241mg
18824-01100ug
Format:1mg/mlinPhosphateBufferedSaline,pH7.2,0.09%sodiumazide.
BACKGROUND
c-mycisanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.ThepeptidesequenceofthemyctagisEQKLISEEDL(Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu).Thec-myctagisapproximately1202Daltonsinatomicmassandincludes10aminoacids.
SPECIFICATIONSUMMARY
Antigen:Aminoacids410-419(EQKLISEEDL)ofhumanmycconjugatedtoKLH.
HostSpecies:Goat
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:20,000.
Detectionofc-myc-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith#18824diluted1:25,000.
Immunocytochemistry:useatadilutionof1:100-1:400.
ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Immunoprecipitation:useat1-4ug/mglysate.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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