ORDERINGINFORMATION
CatalogNo.Size
188221mg
18822-01100ug
Format:1mg/mlinPhosphateBufferedSaline,pH7.2,0.09%sodiumazide.
BACKGROUND
AU5isanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.TheAU5sequencerepresentstheaminoacidsequenceTDFYLK.
SPECIFICATIONSUMMARY
Antigen:AU5(TDFYLK)conjugatedtoKLH.
HostSpecies:Rabbit
Specificity:Antibodywasaffinity-purifiedonthepeptide.
APPLICATIONS
Immunoblotting:useatadilutionof1:1,000-1:20,000.
DetectionofAU5-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith
#18822diluted1:5,000.
Immunocytochemistry:useatadilutionof1:100-1:400.
ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.
Immunoprecipitation:useat1-4ug/mglysate.
Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat2-8oC.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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