ORDERINGINFORMATION

CatalogNo.Size

188201mg

18820-01100ug

Format:1mg/mlinPhosphate-BufferedSaline(PBS),pH7.2,0.09%sodiumazide.

BACKGROUND

AU5isanepitopetagoftenconstructedontheN-orC-terminusofaproteintoallowfordetectionandanalysisoftheprotein.TheAU5sequencerepresentstheaminoacidsequenceTDFYLK.

SPECIFICATIONSUMMARY

Antigen:AU5(TDFYLK)conjugatedtoKLH.

HostSpecies:Goat

Specificity:Antibodywasaffinity-purifiedonthepeptide.

APPLICATIONS

Immunoblotting:useatadilutionof1:1,000-1:20,000.

DetectionofAU5-taggedproteinin200,100,and50ngofE.colilysatecontainingtaggedfusionproteinwith

#18820diluted1:5,000.

Immunocytochemistry:useatadilutionof1:100-1:400.

ELISA:fordetectionuseatadilutionof1:1,000-1:30,000;forcoatinguseatadilutionof1:100-1:500.

Immunoprecipitation:useat1-4ug/mglysate.

Thesearerecommendeddilutions.Endusersshoulddetermineoptimaldilutionsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat2-8oC.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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