BacterialTransglutaminaseMonoclonalAntibody
Transglutaminases(Tgases)aremultifunctionalenzymesthatarewidelydistributedamongmammals,inverte-bratesandplants.StreptomycesmobaraensisTgaseisoneofthemostusefulTgasesduetoitsratherbroadsubstratespecificityandindependenceofCa2+.TheroleofTGaseinthelife-cycleofStreptomycetesisnotfullyunderstood,butitislikelythatitfunctionsinproteincross-linkingthatfortifiestheaerialcellwallandthesporeenvelope.TheproteinstructuresoftheknownbacterialTgasesarequitedifferentfrommammalianTgasesinthattheylacksequencehomologyandhavesmallermolecularmasses.Tgasefrom
S.mobaraensisissecretedasa42.5-kDaprecursorproteinwhichisthenprocessedbytheremovalofa
45amino-acidN-terminalpeptide.
SPECIFICITYThisantibodyrecognizesStreptomycestransglutaminase.Itdoesnotcross-reactwithmammaliantissuetransglutaminase(Tgase2).
Immunoblotting:useat1-10ug/ml.Abandof~38kDaisdetected.
ELISA:useat1-5ug/mlwithStreptomycesTgaseonthesolidphase.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Reconstituteindistilledwater.
Thisproductisstableforatleastone(1)
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SPECIFICATIONSUMMARYAntigen:RecombinantStreptomycesmobaraensistransglutaminase.
Hostspecies:Mouse.AntibodyClass:IgG1.Preservative:None.yearat-20Cto-70C.Reconstituted
productshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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