ORDERINGINFORMATION

Catalognos.:13006-50(50ug)13006-200(200ug)

Format:ProteinA-purifiedantibodyinPBS,pH7.2,50%glycerol,0.09%sodiumazide,.

BACKGROUND

Cytochromep450isahighlyconservedanddiversefamilyofheme-containingproteinsthatfunctioninthemetabolismofnumerousendogenousandexogenoussubstrates.Thehumangenomeencodes57p450genesdividedamong18familieswhichtogetheraccountforthediversityofsubstratesmetabolized.p450istheprimaryconjugatingenzymeinphaseImetabolismanddetoxificationofendogenousandexogenouscompoundsandareprimarilylocalizedtotheinnermembranesofmitochondriaandtheER.Eukaryoticmicrosomalcytochromep450enzymesreceiveelectronsfromtheFAD-andFMN-containingenzymeNADPH:cytochromep450reductase.

SPECIFICATIONSUMMARY

Antigen:NativeratcytochromeP450.

Accessionno.:P05182

GeneID:25086

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Specificity:Thisantibodyrecognizeshuman,mouse,rat,rabbit,canine,Guineapig,horseandmonkeycytochromeP450.

APPLICATIONS

Immunoblotting:Useat1-5ug/ml.Abandof~50-55kDaisdetected.

DetectionofcytochromeP450inhumanlivermicrosomes(Lane2),ratlivermicrosomes(Lane3),mouselivermicrosomes(Lane4),rabbitlivermicrosomes(Lane5).

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidrepeatedfreeze/thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.

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