ORDERINGINFORMATION
CatalogNo.:13042
Format:100ugantigen-affinitypurifiedIgGinTris-citrate/phosphatebuffer,pH7-8,0.09%sodiumazide.
BACKGROUND
AurorakinaseA,alsoknownasserine/threonine-proteinkinase6,isanenzymethatinhumansisencodedbytheAURKAgene.AuroraAisamemberofafamilyofmitoticserine/threoninekinasesassociatedwithimportantprocessesduringmitosisandmeiosis.AuroraAisactivatedbyoneormorephosphorylations,anditsactivitypeaksduringtheG2phasetoMphasetransitioninthecellcycle.AuroraAdysregulationhasbeenassociatedwithhighoccurrenceofcancer.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptiderepresentingaportionoftheproteinencodedwithinexons5and6.
Accessionno.:NP_003591.2
GeneID:6790
HostSpecies:Rabbit
Specificity:ThisantibodyreactswithhumanAurorakinaseA,andtheepitoperecognizedmapstoaregionbetweenresidues75-125ofhumanAurorakinaseA.Basedon100%sequenceidentitythisantibodyispredictedtoreactwithorangutan,gorilla,chimpanzee,andNorthernwhite-cheekedgibbon.
APPLICATIONS
Immunoblotting:1:5,000-1:20,000dilution
DetectionofhumanAuroraAinHeLaandHEPG2wholecelllysateswithcatalogno.13042at0.2ug/ml.Detectionbychemiluminescence.
Immunoprecipitation:1-5ug/mglysate.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableat2-8°Cforone(1)yearfromdateofreceipt.
Forinvitroinvestigationaluseonly.Notintendedforuseintherapeuticordiagnosticprocedures.
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