ADVANCEDRESEARCHTECHNOLOGIES

CatalogNo.:50122

Size:100ugIgGin10mMPBS,pH7.4,1%BSA,lyophilized.

Antigen:HumanplateletsHostSpecies:MouseCloneNumber:WGA-1AntibodyClass:IgG1StABIlizers:1%BSAPreservatives:None

SPECIFICITY

ThisantibodyreactswithGMP-140(P-selectin)onactivatedhumanplatelets;itdoesnotreactwithrestingplatelets.Itdoesnotinterferewithplateletaggregationorplateletadhesion.Itdoesnotcross-reactwithrabbit,rat,porcine,orcanineactivatedplatelets.

DILUTIONINSTRUCTIONS

Dissolvelyophilizedantibodyin50ulofdistilledwater(2.0mg/mlfinalconcentration).

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

APPLICATIONS

ELISA:1-10ug/mlwithimmobilizedantigen.

Flowcytometry:1-10ug/ml

Thrombin-activatedhumanplateletsincubated30minutesatroomtemperaturewith1-2ug#50122per107cells/mlandFITC-labeledsecondaryantibody.

WesternBlot:5-10ug/ml,non-reducingandnon-heatingconditions.

Immunohistochemistry:5-10ug/ml,frozentissuesections.

STORAGEANDSTABILITY

Thisproductdoesnotcontainpreservative.Lyophilizedantibodyisstableat4oCfor2years.Storethereconstitutedsolution(2.0mg/ml)inaliquotsat-20oCforoneyearorat4oCfor6monthsafteradding0.1%sodiumazide.Avoidrepeatedfreeze-thawcycles.

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