ORDERINGINFORMATION
CatalogNo.:56272(cloneGL2A7,ref.GrangerBLetal.1990,JBiolChem265:12036-12403)Format:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography,0.09%sodiumazide,50%glycerol.BACKGROUND
Lysosome-associatedmembraneproteins(LAMP1andLAMP2)aremajorconstituentsofthelysosomalmembrane.Thesetwoproteinshavecloselyrelatedstructureswith37%sequencehomology.Botharetransmembraneglycoproteinslocalizedprimarilyinlysosomesandlateendosomes.LAMP2hasalsobeendetectedattheplasmamembraneofcellsundergoingdifferentiationandactivationandincellsthatsecretelysosomalhydrolases.CellsurfaceLAMP1andLAMP2promoteadhesionofhumanperipheralbloodmononuclearcells(PBMC)tovascularendotheliumwhichsuggeststhattheLAMPproteinsareinvolvedinadhesionofPBMCtositesofinflammation.DefectsinLAMP2areassociatedwithDanondisease.
SPECIFICATIONSUMMARY
Antigen:Gluteraldehyde-fixedmouseliverlysosomesHostSpecies:RatAntibodyClass:IgG1Specificity:Thisantibodyrecognizeshuman,mouse,andrabbitLAMP2(~100-110kDa).Accessionno.:NP_001017959.1GeneID:16784APPLICATIONS
Immunoblotting:Useat1-2ug/ml.Abandof~100-110kDa(glycosylated)isdetected.
Immunofluorescence:Useat1-2ug/ml.ThisantibodylabelslysosomesandlateendosomesincellspermeABIlizedwithsaponin.
Lane1:MWladder,Lane2:20ugHEK293lysate,Lane3:10ug3T3NIHlysate
Thesearerecommendedconcentrations.
Distributionoflysosomesincirculatingendothelialcells(CEC)
Endusershoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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