ORDERINGINFORMATION

BACKGROUND

Fibronectinisa440kDaglycoproteinoftheextracellularmatrixthatbindstointegrins,collagen,fibrin,andheparansulfateproteoglycans.Fibronectinplaysamajorroleincelladhesion,growth,migration,anddifferentiation,anditisimportantforprocessessuchaswoundhealingandembryonicdevelopment.Alteredfibronectinexpression,degradation,andorganizationhasbeenassociatedwithanumberofpathologies,includingcancerandfibrosis.

SPECIFICATIONSUMMARY

Accessionno.:P02751,NP_001293058

APPLICATIONS

ELISA:1-10ug/mlwithimmobilizedantigen.

WesternBlot:1-10ug/ml,reducingornon-reducingconditions.

CellAdhesionInhibitionAssay:0.1-1.0ug/mlon10ug/mlfibronectin-coatedmicrowellsinhibitsadhesionoftrypsinizedNRKcells.

Immunohistochemistry:1-10ug/ml,paraffin-embeddedorfrozentissuesectionswithProteinaseKtreatmentforantigenretrieval.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

Dissolvelyophilizedantibodyin200ulofdistilledwater(finalconcentration2.0mg/ml).

STORAGEANDSTABILITY

Thestocksolution(2.0mg/ml)canbestoredinaliquotsat-20oCfor1yearorcanbestoredat4oCfor6monthsafteradding0.1%sodiumazide.Preparedilutionsofstocksolutionimmediatelybeforeuse.Donotstoredilutedstocksolution.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。