ORDERINGINFORMATION

CatalogNo.:11023(clone5B9.B3)

Format:100uginPBS,pH7.4,purifiedbyProteinGaffinitychromatography.

BACKGROUND

Ubiquitin,asmallproteincomprisedof76aminoacidswithamolecularmassofapproximately10kDa,ishighlyconservedamongeukaryoticspecies.Theprimaryroleofubiquitinistoclearabnormal,foreign,andimproperlyfoldedproteinsbytargetingthemfordegradationbythe26Sproteosome.Ubiquitinationofproteinsisanessentialprocessthatisregulatedbyubiquitin-activatingenzymes,ubiquitin-conjugatingenzymes,andubiquitin-proteinligases.Ubiquitinationalsooccursintheinternalizationanddegradationofplasmamembraneproteins.

SPECIFICATIONSUMMARY

Antigen:NativebovineubiquitinconjugatedtoKLH

HostSpecies:MouseAntibodyClass:IgG2aAccessionno.:NP_776558.1GeneID:281370

SPECIFICITY

Thisantibodyrecognizeshuman,mouse,rat,andbovineubiquitin.Otherspecieshavenotbeeninvestigated.

APPLICATIONS

Immunoblotting:useat1ug/ml.Immunofluorescence:useat10ug/ml.Abandof~10kDaisdetected.

(1)HeLawholecelllysate,(2)Jurkatwholecelllysate.

ELISA:useat1ug/mlwithubiquitinonthesolidphase.

2%formaldehyde-fixedHeLacells,100Xmagnification.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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