Anti-TACE/ADAM17Antibody
TNFissynthesizedasa26kDatypeIImembrane-boundprecursorthatiscleavedbyaconvertasetogeneratesecreted17kDamatureTNF.TNFconvertingenzyme(TACE)hasbeenidentified,andhumanandmouseTACECDNAshavebeencloned.TACEisamembrane-boundmetalloprotease-disintegrininthefamilyofmammalianADAM(foradisintegrinandmetalloprotease).TACEalsoprocessesothercellsurfaceproteins,includingTNFreceptor,TGF,L-selectin,andalpha-cleavageofamyloidproteinprecursor(APP).TACEmRNAisexpressedinavarietyofhumanandmousetissues.
ThissequencediffersfrommouseandratTACEbyoneaminoacid.
Thisantibodyrecognizeshuman,mouse,andratTACE,and80-130kDabandsaredetectedinimmunoblots.Thesebandsmayrepresentmatureprotein,precursor,andglycosylatedTACE.
APPLICATIONSImmunoblotting:useat1ug/ml.
WesternblotanalysisofTACEinHeLa(1,3)andJurkat(2,4)celllysateintheabsence(1,2)orpresence(3,4)ofblockingpeptidewithTACEantibodyat1g/ml.
Positivecontrol:HeLaorJurkatcelllysate.
Immunocytochemistry:useat10ug/ml.
ImmunocytochemicalstainingofTACEinHeLacellswithTACEantibodyat10μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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