CatalogNo.:11007

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

PUMA(p53UpregulatedModulatorofApoptosis)isapro-apoptoticBcl-2familymemberthatencodestwoBH3domain-containingproteins,PUMA-andPUMA-.PUMAproteinsbindBcl-2,localizetomitochondria,andinducecytochromecreleaseandapoptosisinresponsetop53.

Antigen:Syntheticpeptidecorrespondingtoaa180-193ofhuman(accessionnoNP_055232).ThissequenceisidenticalinhumanPUMA-andPUMA-.HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide

ThisantibodyrecognizeshumanandmousePUMA(23kDa).Alowermolecularweightbandat16kDaisdetectedinMOLT4andU937celllysatesandprobablyrepresentsthePUMA-protein.

Immunoblotting:useat2ug/ml.

WesternblotanalysisofPUMAinhuman(H)K562andmouse(M)NIH/3T3celllysateswithPUMAantibodyat2μg/ml.

Positivecontrol:K562celllysate.

Immunocytochemistry:useat1ug/ml.

ImmunocytochemicalstainingofPUMAinK562cellswithPUMAantibodyat1μg/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

美国QED Bioscience 公司（www.qedbio.com）是专业的抗体公司，有20年的历史，是世界著名的抗体生产商。QED生物科技为科研客户提供优质的单克隆和多克隆抗体产品。 是一家提供全面服务的并提供额外服务的公司，能提供超过1500的单克隆和多克隆抗体的用于多领域的研究。