Anti-CAD(CT)Antibody
Celldeathsignalsaretransducedbydeathdomain-containingadaptermoleculesandmembersofthecaspasefamilyofproteases.ThesedeathsignalsfinallycausethedegradationofchromosomalDNAbyactivatedDNase.AmouseDNasethatcausesDNAfragmentationwasidentifiedrecentlyanddesignatedCAD(caspaseactivateddeoxyribo-nuclease).ActivationofCAD/DFF40,whichcausesDNAdegradation,isthehallmarkofapoptoticcelldeath.
Thisantibodyrecognizesfull-lengthmouseCAD(40kDa).
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
APPLICATIONSImmunoblotting:useat2ug/ml.
WesternblotanalysisofCADinmousekidneytissuelysatewithCADantibodyat2μg/ml.
Immunohistochemistry:useat2ug/ml.
ImmunohistochemicalstainingofCADinmousekidneytissuewithCADantibodyat2μg/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:Mouselungtissuelysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
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