CatalogNo.:2015

Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychromatography.

ThemammalianhomologuesofthekeycelldeathgeneCED-4inC.eleganshasbeenidentifiedrecentlyfromhumanandmouseanddesignatedApaf1(forapoptosisprotease-activatingfactor1).Apaf1bindstocytochromec(Apaf2)andcaspase-9(Apaf3),whichleadstocaspase-9activation.Activatedcaspase-9inturncleavesandactivatescaspase-3,oneofthekeyproteasesresponsIBLefortheproteolyticcleavageofmanykeyproteinsinapoptosis.

Apaf1canalsoassociatewithcaspase-4andcaspase-8.Apaf1isubiquitouslyexpressedinhumantissues.

Antigen:Peptidecorrespondingtoaa1158-1177ofhumanApaf1.Thissequence

differsfromthatofmouseApaf1byoneaminoacid.

HostSpecies:Rabbit

Preservatives:0.02%sodiumazide

Thisantibodyrecognizeshuman,mouse,andratApaf1(130kDa).

Immunoblotting:useat1-10ug/ml

WesternblotanalysisofApaf1inhumanhearttissuelysatewithApaf1(CT)antibodyat1μg/mlintheabsence(A)orpresence(B)ofblockingpeptide.

Immunohistochemistry:useat10-20ug/ml

ImmunohistochemistryofApaf1inhumanskintissuewithApaf1(CT)antibodyat20μg/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

Positivecontrol:Humanhearttissuelysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

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