product data sheet

Bacillus anthracis Protective Antigen Monoclonal Antibody

Catalog Nos.: 18720 (C3), 18721

(101), 18722 (102), 18723 (103), 18724

(104), 18725 (105), and 18726 (106).

Size: 200 ug purified antibody in PBS, pH 7.4.

Anthrax toxin produced by Bacillus anthracis is composed of three proteins: Protective Antigen (PA), Edema Factor (EF), and Lethal Factor (LF). Toxicity requires the assembly of all three of these proteins, and this process is initiated when full-length PA binds to the cell surface ATR (anthrax toxin receptor). After binding, PA is enzymatically cleaved to yield a 63 kD fragment which then binds EF and LF, and the complex is internalized.

Antigen: Purified B. anthracis

Protective Antigen (83 kD).

Host Species: Mouse

Antibody Class: IgG1=18720, 18725, 18726; IgG2b=18721, 18722, 18723,

18724

Preservative: 0.1% NaN3

These antibodies recognize different epitopes of the 83kD and 63 kD forms of B. anthracis PA. They do not cross- react with B. anthracis EF, LF, or spore/vegetative surface antigens.

Immunoblotting: use 18721, 18723,

18725, or 18726 at 5 ug/ml.

Sandwich ELISA: use coating antibody (18720 or 18725) at 5 ug/ml; use detect

antibody (18722, 18723, or18726) at 1 ug/ml.

Dilute in PBS or medium which is identical to that used in the assay system.

These antibodies are stable for at least one (1) year at -20oC. Avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures.

QED Bioscience, Inc. 10919 Technology Place, Suite C

San Diego, CA 92127

Toll Free 800.929.2114

Phone 858.675.2405

Fax 858.592.1509

info@qedbio.com

Visit our website for additional product information and to order online.

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